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991.
Aldo S. Pacheco Maria Teresa González Julie Bremner Marcelo Oliva Olaf Heilmayer Jürgen Laudien José M. Riascos 《Helgoland Marine Research》2011,65(3):413-424
Benthic communities show changes in composition and structure across different environmental characteristics and habitats.
However, incorporating species biological traits into the analysis can provide a better understanding of system functioning
within habitats. We compare the functional diversity of macrobenthic communities from a contrasting shallow (15 m) and deep
(50 m) sublittoral soft-sediment habitats in northern Chile, using biological traits analysis. Our aim was to highlight the
biological characteristics responsible for differences between habitats and the implications for ecosystem functioning. Trait
analysis showed that the deep habitat was restricted in providing functionally important biogenic structure and bioturbation
and supports less diverse feeding-related energy pathways. The shallow habitat is characterized by more diverse energy pathways
and a higher potential for matter exchange through bioturbation. We provide support to the predictions of transfer of energy
from the benthos to upper trophic levels in the shallow, which is characterized mainly by normoxia and little organic matter
content in the sediment. In the deep habitat, characterized by hypoxia and more organic matter, energy appears to be transferred
to microbial components. We suggest that trait analysis should be added to the traditional approaches based on species diversity,
because it provides indicators of ecosystem stress. 相似文献
992.
993.
Vassilis Vassiliou Maria Emmanouilidou Andreas Perrakis Evangelia Morou John Vontas Anastasia Tsagkarakou Emmanouil Roditakis 《Insect Science》2011,18(1):30-39
Abstract A comprehensive study on the Bemisia tabaci (biotype B) resistance to neonicotinoid insecticides imidacloprid, acetamiprid and thiamethoxam, and pyrethroid bifenthrin was conducted in Cyprus. The resistance level to eight field‐collected B. tabaci populations was investigated. The activities of enzymes involved in metabolic detoxification and the frequencies of pyrethroid and organophosphates target site resistance mutations were determined. Moderate to high levels of resistance were detected for imidacloprid (resistance factor [RF] 77–392) and thiamethoxam (RF 50–164) while low resistance levels were observed for acetamiprid (RF 7–12). Uniform responses by the Cypriot whiteflies could be observed against all neonicotinoid insecticides. No cross‐resistance between the neonicotinoids was detected as well as no association with the activity of the P450 microsomal oxidases. Only imidacloprid resistance correlated with carboxylesterase activity. Low to extremely high resistance was observed for insecticide bifenthrin (RF 49–1 243) which was associated with the frequency of the resistant allele in the sodium channel gene but not with the activity of the detoxification enzymes. Finally, the F331W mutation in the acetylcholinesterase enzyme ace1 gene was fixed in all B. tabaci populations from Cyprus. 相似文献
994.
995.
Ana Maldonado-Contreras Kate C Goldfarb Filipa Godoy-Vitorino Ulas Karaoz Mónica Contreras Martin J Blaser Eoin L Brodie Maria G Dominguez-Bello 《The ISME journal》2011,5(4):574-579
The human stomach is naturally colonized by Helicobacter pylori, which, when present, dominates the gastric bacterial community. In this study, we aimed to characterize the structure of the bacterial community in the stomach of patients of differing H. pylori status. We used a high-density 16S rRNA gene microarray (PhyloChip, Affymetrix, Inc.) to hybridize 16S rRNA gene amplicons from gastric biopsy DNA of 10 rural Amerindian patients from Amazonas, Venezuela, and of two immigrants to the United States (from South Asia and Africa, respectively). H. pylori status was determined by PCR amplification of H. pylori glmM from gastric biopsy samples. Of the 12 patients, 8 (6 of the 10 Amerindians and the 2 non-Amerindians) were H. pylori glmM positive. Regardless of H. pylori status, the PhyloChip detected Helicobacteriaceae DNA in all patients, although with lower relative abundance in patients who were glmM negative. The G2-chip taxonomy analysis of PhyloChip data indicated the presence of 44 bacterial phyla (of which 16 are unclassified by the Taxonomic Outline of the Bacteria and Archaea taxonomy) in a highly uneven community dominated by only four phyla: Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes. Positive H. pylori status was associated with increased relative abundance of non-Helicobacter bacteria from the Proteobacteria, Spirochetes and Acidobacteria, and with decreased abundance of Actinobacteria, Bacteroidetes and Firmicutes. The PhyloChip detected richness of low abundance phyla, and showed marked differences in the structure of the gastric bacterial community according to H. pylori status. 相似文献
996.
Ali Ouji Maria José Suso Mustapha Rouaissi Raoudha Abdellaoui Mohamed El Gazzah 《Genes & genomics.》2011,33(1):31-38
Seven isozyme systems (Sod, 6-Pgd, Me, Est, Skdh, Fdh and Gdh) representing nine loci were used to study the genetic diversity of nine faba bean populations. Seven loci revealed polymorphic bands and showed the same quaternary structure as that found in several species. They revealed a high number of phenotypes. Indeed, from 3 to 9 phenotypes per locus were investigated in this study. The percentage of polymorphic loci (P = 59.3 %) was higher than that mentioned in the autogamous species (P = 20.3 %) and less than the optimum (P=96 %) indicated for allogamous plants. Total genetic diversity (H T) and within population genetic diversity (H S) were estimated with the isozyme markers. The contribution of among population genetic diversity (D ST) to total genetic diversity was 22%. Enzyme markers pointed out an average inbreeding level for whole population (F IT) and within population (F IS). Within population genetic diversity represents 78% of total diversity. Intra-population genetic diversity (H S = 0.206) was ranged with the respect of allogamous species and was clearly higher than that of among population genetic diversity (D ST = 0.057) indicating an out-crossing predominance in the studied populations. The expected heterozygosity was higher than that observed heterozygosity at the allogamous species was confirmed in this study. Although, the mean estimated gene flow was less than 1(Nm=0.814), the dendrogram based on Nei’s genetic distance of the 9 populations using UPGMA method showed some genetic drift between populations. 相似文献
997.
Elena Pompili Cinzia Fabrizi Stefania Lucia Nori Barbara Panetta Maria Concetta Geloso Valentina Corvino Fabrizio Michetti Lorenzo Fumagalli 《The journal of histochemistry and cytochemistry》2011,59(3):302-311
In the nervous system, protease-activated receptors (PARs), which are activated by thrombin and other extracellular proteases, are expressed widely at both neuronal and glial levels and have been shown to be involved in several brain pathologies. As far as the glial receptors are concerned, previous experiments performed in rat hippocampus showed that expression of PAR-1, the prototypic member of the PAR family, increased in astrocytes both in vivo and in vitro following treatment with trimethyltin (TMT). TMT is an organotin compound that induces severe hippocampal neurodegeneration associated with astrocyte and microglia activation. In the present experiments, the authors extended their investigation to microglial cells. In particular, by 7 days following TMT intoxication in vivo, confocal immunofluorescence revealed an evident PAR-1-related specific immunoreactivity in OX-42-positive microglial cells of the CA3 and hilus hippocampal regions. In line with the in vivo results, when primary rat microglial cells were treated in vitro with TMT, a strong upregulation of PAR-1 was observed by immunocytochemistry and Western blot analysis. These data provide further evidence that PAR-1 may be involved in microglial response to brain damage. 相似文献
998.
999.
Graça Soveral Catarina Prista Teresa F. Moura Maria C. Loureiro‐Dias 《Biology of the cell / under the auspices of the European Cell Biology Organization》2011,103(1):35-54
In yeast, the presence of orthodox aquaporins has been first recognized in Saccharomyces cerevisiae, in which two genes (AQY1 and AQY2) were shown to be related to mammal and plant water channels. The present review summarizes the putative orthodox aquaporin protein sequences found in available genomes of yeast and filamentous fungi. Among the 28 yeast genomes sequenced, most species present only one orthodox aquaporin, and no aquaporins were found in eight yeast species. Alignment of amino acid sequences reveals a very diverse group. Similarity values vary from 99% among species within the Saccharomyces genus to 34% between ScAqy1 and the aquaporin from Debaryomyces hansenii. All of the fungal aquaporins possess the known characteristic sequences, and residues involved in the water channel pore are highly conserved. Advances in the establishment of the structure are reviewed in relation to the mechanisms of selectivity, conductance and gating. In particular, the involvement of the protein cytosolic N‐terminus as a channel blocker preventing water flow is addressed. Methodologies used in the evaluation of aquaporin activity frequently involve the measurement of fast volume changes. Particular attention is paid to data analysis to obtain accurate membrane water permeability parameters. Although the presence of aquaporins clearly enhances membrane water permeability, the relevance of these ubiquitous water channels in yeast performance remains obscure. 相似文献
1000.
Chiara Lombardi Maria Cristina Gambi Claudio Vasapollo Paul Taylor Silvia Cocito 《Zoomorphology》2011,130(2):135-145
Colonies of the cheilostome bryozoan Schizoporella errata were grown at a site near Ischia Island (Tyrrhenian Sea, Italy) where volcanogenic CO2 emissions lower seawater pH to 7.76, simulating levels of ocean acidification predicted for the end of the present century.
Compared with colonies from a control site (mean pH = 8.09), putative defensive polymorphs (avicularia) were significantly
fewer, and retarded growth of zooidal basal and lateral walls was evident at the low pH site. The lower proportion of avicularia
suggests a switch in resource allocation away from defence to favouring rapid growth. In addition, corrosion of the skeleton
was observed in both new and old zooids at the low pH site, and feeding zooids were slightly smaller but had larger orifices
for the protrusion of feeding lophophores. These findings corroborate previous studies demonstrating potential dissolution
of carbonate skeletons in low pH seawater, while providing new insight into the possible ability of colonial species to respond
to ocean acidification by adjusting resource allocation between zooids of different types. 相似文献